Establishment of experimental rat model of type 2 diabetes with streptozotocin and high-glucose-high-fat diet

Objective:To establish the experimental rat model of type 2 diabetes by streptozotocin and high-glucose-high-fat diet and observe its stability. Methods:60 SD male rats were randomly divided into four groups:control group,streptozotocin group(STZ group),high-glucose-high-fat feeding group (H.E group)and high-glucose-high-fat feeding+STZ group (H.E+STZ group). STZ group and H.E + STZ group were intraperitoneal injected with STZ 35 mg / kg. 4 weeks later,we measured and statistically analyzed the changes of body weight,blood insulin level,fasting blood glucose level,blood lipid level,insulin sensitivity index (ISI)and area under curve and insulin sensitivity index (AUC)of hamsters of every group at different time points. Results:The fasting plasma insulin, Serum triglyceride(TG),total cholesterol(TC),ISI of H.E group and H.E+STZ group significantly increased(P 0.01), the Fasting blood glucose and AUC of STZ group and H.E+STZ group increased obviously (P 0.01). Compared with the control group and H.E group,the Fasting plasma insulin and TG,TC,ISI of H.E+STZ group injected with STZ for four weeks increased significantly (P 0.01). Conclusion:High-glucose-high-fat diet significantly decreased insulin sensitivity index,and the type 2 diabetes rat models were established by high-glucose-high-fat diet combind with low dose injection of STZ with high success rate,effective stability and shorter model-making time.