Study of blood-spinal cord barrier disruption after spinal cord injury in rats

Objective:Investigate the mechanism of blood spinal cord barrier disruption after spinal cord injury.Method:Thirty five male adult Wistar rats(300～350g) were randomly assigned to this study,there were one control group and six spinal cord injury groups(acording to the time of post injury,4h,6h,12h,24h,48h and 72h).Each group contained 5 rats and New York University (NYU) Spinal Cord Injury Model was utilized to create the spinal cord injury.The immunoexpressior changes of immunglobularprotein G(IgG),c fragment of complement3 (C3c) in different time after spinal cord injury were evaluated utilizing immunohistochemistry method.Result:After spinal cord injury, there was a marked chang in the immunoexpressior of IgG and C3c in the impact site, near the impact site and spinal cord microvascular.Conclusion:After spinal cord injury,IgG and C3c may be important factors of barrier disruption and related to neuron secondary injury.