Effects of Microcystin-LR on Antioxidant Enzymes and Reactive Oxygen Species in Tilapia Fish

This paper firstly focused on the variation of reactive oxygen species(ROS) and antioxidant enzymes,such as superoxide dismutase(SOD) and catalase(CAT),to tilapia in exposed to microcystin-LR(MC-LR) by intraperitoneal.In this experiment,five treatments(control,50,100,250,500 μg·kg-1 MC-LR) were designed,the value of ROS,SOD,CAT were detected at time 2,12,24,36,84 h.The results showed that surplus ROS were generated in liver in exposed to MC-LR by intraperitoneal.SOD and CAT played a different role in eliminating the surplus ROS,dynamic process of SOD showed that it had a sudden increase in 36 h,and dynamic process of CAT showed that it had two peaks respectively in 24 h and 84 h.The experiment was to certify that antioxidant enzymes could be used as biomarkers in monitoring the toxic effect of tilapia in exposed to MC in liver,due to their dose and time-response effect variation.The experiment makes a progress in studying the toxic mechanism of microcystin in terms of the antioxidant defence system.