[Determination of octafluoropropane content in human albumin micropheres loaded with octafluoropropane].

To develop a method for determinating the octafluoropropane (OFP) content in human albumin micropheres loaded with OFP.OFP content in the loaded human albumin micropheres was determined by means of gas chromatography coupled with mass spetrum/selected ion monitoring (GC-MS/SIM).The calibration curve was linear within the concentration range of 40.92-16,368 nmol/L (R(2)=0.9999). The limit of detection was 8.18 nmol/L, and the recovery rate was (100.98-/+1.4)% (204 nmol/L), (102.63-/+2.29)% (2,046 nmol/L) and (97.5-/+2.2)% (8,184 nmol/L), respectively. The intra-day and inter-day RSDs were 0.6%-2.0% and 0.86%-2.3%, respectively.The method of GC-MS/SIM is accurate, sensitive, precise and applicable for OFP determination in the microspheres.