Spider silk is the natural protein fiber of Semi-crystalline biopolymer consisting of(GPGXX)n /(GPGQQ) n , An/(GA)n,(GGX) n etc motifs and have diversiform molecular structures,mechanical properties and biological functions.Spider silk is an attractive biomaterial for its excellent mechanical properties with the unusual combination of high stiffness, toughness, strength, and extensibility which rarely observed in synthetic high-performance fibers.This paper reviewed the compositions, structures, mechanical properties, weaving mechanism and application prospect of spider silk.