Culture of Fibroblast from Goat Skin Tissue Stored at Different Temperature

In order to enrich the access to obtain fibroblast from goat, in this experiment, we got out the goat ear skin tissues, and conserved at 4℃ and 20℃ for some time, then the fibroblast were primary cultured and subcultured or were frozen-thawed and then subcultured. The results showed as followed: compared with the control group(fresh skin)which cellular adherent needed 2d in primary culture and 8d in subculture to 80% confluence, after stored at 20℃ for 12 or 24h, time had delayed in primary cultured cellular adherent(3~6d) and subcultured to 80% confluence(12~14d). After 24 and 48h at 4℃, cell anchorage-dependent need 3d in primary culture and 10d to 80% confluence. Compared with the time to 80% confluence(3d), the fibroblast in the first and second subculture which stored at 20℃and 4℃ reached to 80% confluence in the 3d or 4d after subcultured. The frozen-thawed and then subcultured cells which came from the control group needed 4d to 80% confluence. However, the fibroblast stored at 20℃and 4℃ needed 4 or 5d to 80% confluence, no matter how long it stored. All the results indicated that, even kept at 4 or 20℃ for 1~2d after the goat dead, the skin fibroblasts could also be obtained which was similar to the fresh skin fibroblasts.