Evaluation of clenbuterol-induced changes in blood biochemical parameters in white mice

Clenbuterol is a β 2 -adrenergic agonist which is illegally used in animal production as a promoting agent.The accumulation of clenbuterol in edible tissues has potentially adversed effects that can induce to long-term metabolism disorders after clenbuterol withdrawal.Purpose of this study is to analysis effects of clenbuterol residues on some blood parameters in white mice.68 mice were divided into 3 groups: control group (n = 27), clenbuterol group A (CA) (n = 20) and clenbuterol group B (CB) (n = 21).Clenbuterol groups were received clenbuterol 2 µg/kg orally once daily.After 8 weeks, mice in group A discontinued receiving clenbuterol and mice in group B continued receiving clenbuterol until 16 weeks.Liver, kidney and blood samples were collected at week 8 and week 16 for biochemistry parameter tests: plasma glucose, triglyceride, total cholesterol, total protein, creatinine concentrations; plasma AST and ALT activities.Results showed that oral clenbuterol administration at dosage 2 µg/kg once daily for 8 weeks significantly decreased ALT activities and elevated glucose concentrations.Clenbuterol-induced dyslipidemia might reverse after withdrawing using clenbuterol while hyperglycemia might potentially make a progress.