Ecotoxicological investigations of extremely acidic mining lakes using bioassays suitable for testing at low pH

In this investigation different bioassays were used to detect the physiological and genotoxic effects of water samples of mining lakes. Especially bioassays with higher plants were used like the duckweed bioassay with Lemna minor, the cress bioassay with Lepidium sativum, the Tradescantia‐micronucleus bioassay and the Arabidopsis bioassay. In addition the algae‐motility bioassay with Euglena gracilis were used. All these bioassays were found to be suitable to investigate mining lake samples of extreme acidic character. Four mining lakes (ML 107, ML 111, ML 117, ML F) were selected. They show equally low pH‐values, but differ in size, age, hydro‐chemical and biological state. The results demonstrated that it is possible to differentiate the mining lakes based on the results of bioassays. Samples of ML 107 and 111 showed significant physiological and genotoxic effects. Water samples of both lakes caused severe damages and decreased growth at the same dilution level in all plant bioassays. ML F causes different effects on bioassays. The highest negative effect was observed on root development of L. sativum. ML 117 did not show any effects on duckweed, algae, Tradescantia or Arabidopsis bioassays. There was only a slight effect on the root development in the cress bioassay.