谷胱甘肽
化学
胱氨酸
半胱氨酸
硫辛酸
硫醇
氧化还原
半胱胺
吸光度
谷胱甘肽二硫化物
硫辛酸
无机化学
生物化学
抗氧化剂
色谱法
酶
出处
期刊:European journal of biochemistry
[Wiley]
日期:1967-10-01
卷期号:2 (3): 327-331
被引量:255
标识
DOI:10.1111/j.1432-1033.1967.tb00142.x
摘要
Thiol-disulphide exchange between glutathione (GSH) and cystine (CSSC) or oxidised glutathione (GSSG) and cysteine (CSH) has been followed by assaying GSH and GSSG specifically at low pH during the reaction. Velocity and equilibrium constants have been derived. From the latter and the known standard oxidation-reduction potential (E0′) of GSH/GSSG, E0′ (pH 7) of CSH/CSSC is found to be -0.22 volt. The course of the reaction of both these pairs with dihydrolipoic acid/lipoic acid has been inferred from absorbance changes at 330 mμ. Results confirm that their E0′ values are considerably more positive than for the dihydrolipoic acid/lipoic acid system.
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