光热治疗
表面改性
纳米棒
癌细胞
表皮生长因子受体
胶体金
材料科学
生物物理学
纳米技术
细胞
抗体
化学
癌症研究
纳米颗粒
受体
癌症
医学
生物化学
免疫学
生物
物理化学
内科学
作者
Kathleen Black,Ji Yi,José G. Rivera,Daria C. Zelasko-Leon,Phillip B. Messersmith
出处
期刊:Nanomedicine
日期:2013-01-01
卷期号:8 (1): 17-28
被引量:191
摘要
A novel biomimetic strategy was employed for presenting antibodies on gold nanorods (NRs) to target growth factor receptors on cancer cells for use in photothermal therapy.Polydopamine (PD) was polymerized onto gold NRs, and EGF receptor antibodies (anti-EGFR) were immobilized onto the layer. Cell-binding affinity and light-activated cell death of cancer cells incubated with anti-EGFR-PD-NRs were quantified by optical imaging.PD was deposited onto gold NRs, and antibodies were bound to PD-coated NRs. Anti-EGFR-PD-NRs were stable in media, and were specifically bound to EGFR-overexpressing cells. Illumination of cells targeted with anti-EGFR-PD-NRs enhanced cell death compared with nonirradiated controls and cells treated with antibody-free NRs.PD facilitates the surface functionalization of gold NRs with biomolecules, allowing cell targeting and photothermal killing of cancer cells. PD can potentially coat a large variety of nanoparticles with targeting ligands as a strategy for biofunctionalization of diagnostic and therapeutic nanoparticles.
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