医学
肌酐
肾功能
生物标志物
肾
急性肾损伤
透析
趋化因子
泌尿科
病理生理学
缺血
肾脏疾病
内科学
病理
炎症
生物
生物化学
作者
Roshni R. Molls,Vladimir Savransky,Manchang Liu,Shannon Bevans,Tulsi Mehta,Rubin M. Tuder,Landon S. King,Hamid Rabb
出处
期刊:American Journal of Physiology-renal Physiology
[American Physiological Society]
日期:2006-05-01
卷期号:290 (5): F1187-F1193
被引量:114
标识
DOI:10.1152/ajprenal.00342.2005
摘要
Renal ischemia-reperfusion injury (IRI) is the leading cause of acute kidney injury [AKI; acute renal failure (ARF)] in native kidneys and delayed graft function in deceased donor kidney transplants. Serum creatinine rises late after renal IRI, which results in delayed diagnosis. There is an important need to identify novel biomarkers for early diagnosis and prognosis in renal IRI. Given the inflammatory pathophysiology of renal IRI, we used a protein array to measure 18 cytokines and chemokines in a mouse model of renal IRI at 3, 24, and 72 h postischemia. A rise in renal keratinocyte-derived chemokine (KC) was the earliest and most consistent compared with other molecules, with 3-h postischemia values being 9- and 13-fold greater than sham and normal animals, respectively. Histological changes were evident within 1 h of IRI but serum creatinine only increased 24 h after IRI. With the use of an ELISA, KC levels in serum and urine were highest 3 h postischemia, well before a significant rise in serum creatinine. The human analog of KC, Gro-alpha, was markedly elevated in urine from humans who received deceased donor kidney transplants that required dialysis, compared with deceased donor kidney recipients with good graft function and live donor recipients with minimal ischemia. Measurement of KC and its human analog, Gro-alpha, could serve as a useful new biomarker for ischemic ARF.
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