免疫球蛋白E
蛋白质A
化学
蛋白质G
抗体
琼脂糖
蛋白质A/G
洗脱
分子生物学
免疫球蛋白M
免疫球蛋白G
色谱法
酶
免疫学
生物
生物化学
融合蛋白
重组DNA
基因
作者
Zhikang Peng,F. Estelle,Richard K. Simons,Allan B. Becker
标识
DOI:10.1016/0022-1759(91)90335-d
摘要
We studied the binding of dog immunoglobulins G, A, M and E to protein A and protein G. Passive cutaneous anaphylaxis (PCA) testing was used for the measurement of dog IgE and enzyme-linked immunosorbent assays (ELISA) were used for the measurements of dog IgG, IgA and IgM. Protein A from lyophilized cells of Staphylococcus aureus bound 97% of IgE, 98% of IgG, 81% of IgA, and 97% of IgM. Protein A-Sepharose CL-4B bound 87% of IgE, 100% of IgG and IgA, and 98% of IgM. In a stepwise elution with varying pH, a small amount of IgE was eluted at pH 5 and pH 6 and all the remaining Igs were eluted at pH 3 from the protein A column. In contrast to protein A, dog IgE was not bound to Protein G-Sepharose, while 100% of IgG, 95% of IgA, and 44% of IgM were bound to Protein G-Sepharose.
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