小火焰菌属
萜烯
倍半萜
生物合成
酶
化学
生物化学
癸烷
有机化学
立体化学
食品科学
蘑菇
作者
Huiying Han,Ping Zhang,Zhiping Xie,Jianzhao Qi,Pengchao Wang,Li Chien Chang,Zheyong Xue,Ruibo Wu,Chengwei Liu
标识
DOI:10.1021/acs.jafc.4c01636
摘要
Flammulina velutipes, a popular edible mushroom, contains sesquiterpenes with potential health benefits. We characterized 12 sesquiterpene synthases and one P450 enzyme in F. velutipes using Aspergillus oryzae as a heterologous expression system, culminating in the biosynthesis of 16 distinct sesquiterpene compounds. An enzyme encoded by the axeB gene responsible for the synthesis of the spiro [4.5] decane compound axenol was discovered, and the mechanism of spirocycle formation was elucidated through quantum mechanical calculations. Furthermore, we delineated the role of a P450 enzyme colocated with AxeB in producing the novel compound 3-oxo-axenol. Our findings highlight the diverse array of sesquiterpene skeletons and functional groups biosynthesized by these enzymes in F. velutipes and underscore the effectiveness of the A. oryzae system as a heterologous host for expressing genes in the Basidiomycota genome. These insights into the biosynthesis of bioactive compounds in F. velutipes have significant implications for functional food and drug development.
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