克鲁维酵母
玉米芯
调节器
克鲁维酵母
发酵
酵母
水解
化学
乙醇发酵
转录因子
生物化学
酿酒酵母
细胞生物学
生物
基因
有机化学
原材料
作者
Kehui Zhang,Zaiyong Jiang,Xingjiang Li,Dongmei Wang,Jiong Hong
标识
DOI:10.1016/j.biortech.2024.130627
摘要
Overexpression of a gene with unknown function in Kluyveromyces marxianus markedly improved tolerance to lignocellulosic biomass-derived inhibitors. This overexpression also enhanced tolerance to elevated temperatures, ethanol, and high concentrations of NaCl and glucose. Inhibitor degradation and transcriptome analyses related this K. marxianus Multiple Stress Resistance (KmMSR) gene to the robustness of yeast cells. Nuclear localization and DNA-binding domain analyses indicate that KmMsr is a putative transcriptional regulator. Overexpression of a mutant protein with deletion in the flexible region between amino acids 100 and 150 further enhanced tolerance to multiple inhibitors during fermentation, with ethanol production and productivity increasing by 36.31 % and 80.22 %, respectively. In simultaneous saccharification co-fermentation of corncob without detoxification, expression of KmMSR with the deleted flexible region improved ethanol production by 5-fold at 42 °C and 2-fold at 37 °C. Overexpression of the KmMSR mutant provides a strategy for constructing robust lignocellulosic biomass using strains.
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