Quantitative susceptibility atlas construction in Montreal Neurological Institute space: towards histological-consistent iron-rich deep brain nucleus subregion identification

Iron-rich deep brain nuclei (DBN) of the human brain are involved in various motoric, emotional and cognitive brain functions. The abnormal iron alterations in the DBN are closely associated with multiple neurological and psychiatric diseases. Quantitative susceptibility mapping (QSM) provides the spatial distribution of the magnetic susceptibility of human brain tissues. Compared to traditional structural imaging, QSM provides superiority for imaging the iron-rich DBN owing to the susceptibility difference existing between brain tissues. In this study, we constructed a Montreal Neurological Institute (MNI) space unbiased QSM human brain atlas via group-wise registration from 100 healthy subjects aged 19-29 years. The atlas construction process was guided by hybrid images that were fused from multi-modal magnetic resonance images (MRI). We named it as Multi-modal-fused magnetic Susceptibility (MuSus-100) atlas. The high-quality susceptibility atlas provides extraordinary image contrast between iron-rich DBN with their surroundings. Parcellation maps of DBN and their subregions that are highly related to neurological and psychiatric pathology were then manually labeled based on the atlas set with the assistance of an image border-enhancement process. Especially, the bilateral thalamus was delineated into 64 detailed subregions referring to the Schaltenbrand-Wahren stereotactic atlas. To our best knowledge, the histological-consistent thalamic nucleus parcellation map is well defined for the first time in the MNI space. Compared with existing atlases that emphasizing DBN parcellation, the newly proposed atlas outperforms on the task of atlas-guided individual brain image DBN segmentation both in accuracy and robustness. Moreover, we applied the proposed DBN parcellation map to conduct detailed identification of the pathology-related iron content alterations in subcortical nuclei for Parkinson's Disease (PD) patients. We envision that the MuSus-100 atlas can play a crucial role in improving the accuracy of DBN segmentation for the research of neurological and psychiatric disease progress and also be helpful for target planning in deep brain stimulation surgery.