Enhancing diabetic wound healing with a pH-responsive nanozyme hydrogel featuring multi-enzyme-like activities and oxygen self-supply

Diabetic wound treating remains a challenging due to bacterial infections, oxidative stress, tissue hypoxia , and high glucose levels. Herein, a multi-enzyme-like activities nanocomposite (Mo,Fe/Cu,I-Ag@GOx) was designed and anchored to a multifunctional fluorescence hydrogel. The nanozyme gel, loaded with glucose-oxidase (GOx), exhibits intrinsic GOx , peroxidase (POD)-, oxidase (OXD)-, catalase (CAT)- and superoxide dismutase (SOD)-like activities with pH-switchable glucose-initiated cascade reaction for diabetic wound healing . In the first cascade-reaction, initiated by GOx , the nanozyme gel catalyzes glucose and O 2 into gluconic acid and H 2 O 2 to further generate superoxide anion radical (O 2 ·- ) and hydroxyl radicals (·OH) to eradicate bacteria. In the second cascade-reaction, as the wound pH changes alkalescent microenvironment, the nanozyme gel simulates SOD to transform O 2 ·- into O 2 and H 2 O 2 , and then decomposes endogenous and exogenous H 2 O 2 into O 2 via CAT-like activity to reduce oxidative stress and alleviate hypoxia . The gel by calcium ion (Ca 2+ ) cross-linked sodium alginate (SA) and chitosan (CS) containing nanozyme was constructed with injectability, adhesion and fluorescence properties, as well as beneficial biocompatible. Importantly, the water/alcohol solubility of the nanozyme gel allows it to be used as a dressing without causing secondary injury to the wound. The multifunctional fluorescence hydrogel exhibits efficiently promote pro-angiogenesis and bacteria-infected wound healing.