Exo-miR-144-3p as a promising diagnostic biomarker for depressive symptoms in heart failure

小桶 小RNA 生物标志物 基因 实时聚合酶链反应 基因表达 生物信息学 肿瘤科 心力衰竭 逆转录聚合酶链式反应 内科学 基因本体论 生物 医学 遗传学
作者
Ruting Wang,Kai Huang,Yuehua Feng,Jiahao Duan,Hangfeng Ying,Qianyuan Shi,Yi Zhang,Riyue Jiang,Ling Yang
出处
期刊:Neurobiology of Disease [Elsevier BV]
卷期号:: 106415-106415 被引量:6
标识
DOI:10.1016/j.nbd.2024.106415
摘要

The prevalence of depression is higher in heart failure (HF) patients. Early screening of depressive symptoms in HF patients and timely intervention can help improve patients' quality of life and prognosis. This study aims to explore biomarkers for disease diagnosis by examining the expression profile of serum exosomal miRNAs in HF patients with depressive symptoms. Serum exosomal RNA was isolated and extracted from 6 HF patients with depressive symptoms (HF-DS) and 6 HF patients without depressive symptoms (HF-NDS). High-throughput sequencing was performed to obtain miRNA expression profiles and target genes were predicted for the screened differentially expressed miRNAs. Biological functions of the target genes were analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Subsequently, we collected serum exosomal RNAs from HF-DS (n = 20) and HF-NDS (n = 20). The differentially expressed miRNAs selected from the sequencing results were validated using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Finally, the diagnostic efficacy of the differentially expressed exosomal miRNAs in predicting HF with depressive symptoms was evaluated by using receiver operating characteristic (ROC) curves. A total of 19 significantly differentially expressed exosomal miRNAs were screened by high-throughput sequencing, consisting of 12 up-regulated and 7 down-regulated exosomal miRNAs. RT-qPCR validation demonstrated that the expression level of exo-miR-144-3p was significantly down-regulated in the HF-DS group, and the expression levels of exo-miR-625-3p and exo-miR-7856-5p were significantly up-regulated. The expression level of exo-miR-144-3p was significantly negatively correlated with the severity of depressive symptoms in HF patients, and the area under the curve (AUC) of exo-miR-144-3p for diagnosing HF with depressive symptoms was 0.763. In this study, we examined the serum exosomal miRNA expression profiles of HF patients with depressive symptoms and found that lower levels of exo-miR-144-3p were associated with more severe depressive symptoms. Exo-miR-144-3p is a potential biomarker for the diagnosis of HF with depressive symptoms.
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