聚电解质
凝聚
化学工程
背景(考古学)
逐层
等温滴定量热法
材料科学
聚合物
透明质酸
化学
高分子化学
图层(电子)
纳米技术
色谱法
生物化学
复合材料
古生物学
遗传学
工程类
生物
作者
Tomasz Urbaniak,Paweł J. Piszko,Dana Kubies,Zuzanna Podgórniak,Ognen Pop‐Georgievski,Tomáš Riedel,Konrad Szustakiewicz,Witold Musiał
标识
DOI:10.1016/j.ejpb.2023.10.023
摘要
The modification of biomaterial surfaces has become increasingly relevant in the context of ongoing advancements in tissue engineering applications and the development of tissue-mimicking polymer materials. In this study, we investigated the layer-by-layer (LbL) deposition of polyelectrolyte multilayer protein reservoirs consisting of poly-l-lysine (PLL) and hyaluronic acid (HA) on the hydrophobic surface of poly(glycerol sebacate) (PGS) elastomer. Using the methods of isothermal titration calorimetry and surface plasmon resonance, we systematically investigated the interactions between the polyelectrolytes and evaluated the deposition process in real time, providing insight into the phenomena associated with film assembly. PLL/HA LbL films deposited on PGS showed an exceptional ability to incorporate bone morphogenetic protein-2 (BMP-2) compared to other growth factors tested, thus highlighting the potential of PLL/HA LbL films for osteoregenerative applications. The concentration of HA solution used for film assembly did not affect the thickness and topography of the (PLL/HA)10 films, but had a notable impact on the hydrophilicity of the PGS surface and the BMP-2 release kinetics. The release kinetics were successfully described using the Weibull model and hyperbolic tangent function, underscoring the potential of these less frequently used models to compare the protein release from LbL protein reservoirs.
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