Mutation-Induced Rigidity in the Fyn SH2 Domain Enhances pY-Binding Affinity at the Cost of Peptide Specificity

Interactions between SH2 domains and tyrosine-phosphorylated (pY) peptides are essential for cellular signaling. While structural studies have revealed how triple-point Fyn SH2 mutants achieve ultra-high pY-peptide affinity, the dynamic consequences...