Solid-state nanopore detection of metal ions via a DNAzyme–Catalytic Hairpin Assembly reaction

Based on the indirect transduction strategy (ITS), we utilize a DNAzyme-CHA reaction to transduce lead(II), which cannot be directly detected by a glass nanopore, into controllable CHA products. This enables the accurate recognition of lead(II). The signal amplification method significantly enhances the resolution of glass nanopores and is expected to broaden their application scope.