Small-Molecule Binding-Guided Nucleic Acid Amplification and Its Ultrasensitive Electrochemical Detection of Aflatoxin B1: A Split Aptamer Ligation Chain Reaction via Click Chemistry

ng/mL, a low detection limit of 16.7 pg/mL, and a high specificity toward one group discrimination. It also successfully detected the target of interest in complex matrices. This study provides a novel alternative for connecting small molecules with NAA and for small molecule biosensing, which is free of enzymatic restrictions and the elaborate design of strand displacement.