CRISPR/dCas9—surface-enhanced Raman scattering for the detection of drug resistance gene macB

Abstract Antibiotics have brought many benefits to public health systems worldwide since they were first used in the last century, yet with the overuse of antibiotics in clinical care and livestock farming, new public health issues have arisen.In our previous experiments, we found that the MacB gene in bovine raw milk ranked first among many drug resistance genes.In this paper, we present a strategy for the highly sensitive detection of the drug resistance gene MacB by combining clusters regularly interspaced short palindromic repeats (CRISPR) with surface-enhanced Raman scattering (SERS) technology.This strategy integrates the specific gene identification capabilities of the CRISPR system, the ultra-sensitivity of SERS, the chemometric algorithm analysis and the simple separation properties of nanoparticles, eliminating the need for complex steps such as purification and gene amplification, offering the advantages of rapid, ultra-sensitive and highly specific detection, thus enabling more effective tongue safety. Keywords: CRISPR/dCas9; Surface-Enhanced Raman Scattering; drug resistance gene MacB; gold nanoparticles.