磷钨酸
谷胱甘肽
抗坏血酸
化学
检出限
催化作用
电子受体
氧化还原
光电阴极
选择性
半胱氨酸
线性范围
接受者
电子转移
光化学
无机化学
色谱法
电子
生物化学
酶
物理
量子力学
食品科学
凝聚态物理
作者
Yifan Jiang,Huilan Zhang,Meizhu Xu,Fang Luo,Cuiying Lin,Bin Qiu,Zhenyu Lin,Jiang Zhou,Jian Wang
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:148 (13): 2983-2991
被引量:2
摘要
A novel photoelectrochemical (PEC) sensor was constructed for the highly sensitive detection of reduced glutathione (GSH) based on the multiple catalytic properties of phosphotungstic acid (PTA). In this work, the catalytic properties of PTA were applied to PEC sensing for the first time and interpreted in detail. First, PTA as an electron acceptor can inhibit the complexation of photogenerated electron-hole pairs in p-Cu2O, thus significantly increasing the photogenerated current of p-type semiconductor material Cu2O. Secondly, when GSH is oxidized to oxidized glutathione (GSSG) by photogenerated holes on the photocathode, PTA is able to reduce GSSG to GSH by transferring protons, forming a redox cycle regeneration process of GSH. Finally, the relatively large amount of PTA in the background solution was able to pre-oxidize interfering substances such as L-cysteine and ascorbic acid, which improved the selectivity of the method. Under the optimal experimental conditions, the linear range of the PEC sensor response to GSH was 0.050-100 nmol L-1, with a detection limit as low as 0.017 nmol L-1 (S/N = 3), which can be applied to the detection of GSH content in cell lysate samples.
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