生物钟
细胞生物学
生物
时钟
细胞生长
细胞周期
下调和上调
调节器
内科学
昼夜节律
内分泌学
细胞
医学
基因
遗传学
作者
Liang Huang,Huatang Yuan,Shengjie Shi,Xiangrong Song,Lutong Zhang,Xiaoge Zhou,Lei Gao,Wei Pang,Gongshe Yang,Guiyan Chu
标识
DOI:10.1186/s40104-023-00884-7
摘要
Clock circadian regulator (CLOCK) is a core factor of the mammalian biological clock system in regulating female fertility and ovarian physiology. However, CLOCK's specific function and molecular mechanism in porcine granulosa cells (GCs) remain unclear. In this study, we focused on CLOCK's effects on GC proliferation.CLOCK significantly inhibited cell proliferation in porcine GCs. CLOCK decreased the expression of cell cycle-related genes, including CCNB1, CCNE1, and CDK4 at the mRNA and protein levels. CDKN1A levels were upregulated by CLOCK. ASB9 is a newly-identified target of CLOCK that inhibits GC proliferation; CLOCK binds to the E-box element in the ASB9 promoter.These findings suggest that CLOCK inhibits the proliferation of porcine ovarian GCs by increasing ASB9 level.
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