A bilayer mupirocin/bupivacaine-loaded wound dressing based on chitosan/poly (vinyl alcohol) nanofibrous mat: Preparation, characterization, and controlled drug release

An infected skin wound caused by external injury remains a serious challenge. Electrospun drug-loaded nanofibers with antibacterial properties based on biopolymers have been widely explored for wound healing. In this study, the double-layer CS/PVA/mupirocin (CPM) + CS/PVA/bupivacaine (CPB) mats were prepared by electrospinning method (20 % polymer weight) and then crosslinked with glutaraldehyde (GA) to optimize the water-resistant and biodegradation properties for wound dressing applications. The morphology of mats was characterized as defect-free and interconnected nanofibers by Scanning Electron Microscope (SEM) and Atomic Force Microscopy (AFM). Fourier Transform Infrared Spectrometry (FTIR) analysis also assessed the chemical structural properties. The porosity, surface wettability, and swelling degree of the dual-drug loaded mats were improved by about 20 %, 12°, and 200 % of the CS/PVA sample to provide a moist environment for efficient wound breathing and repairing. This highly porous mat facilitated the wound exudates absorption and air permeability excellently, reducing the chance of bacterial infections by inhibiting the growth of S. aureus bacterial colonies with a zone of 71.3 mm diameter. In vitro drug release results showed a high-burst release of 80 % and a continuous release profile for bupivacaine and mupirocin, respectively. MTT assay and in vivo tests indicated >90 % of cell viability and improvement in cell proliferation. It triply accelerated wound closure compared to the control group, reaching nearly full closure after 21 days as a potential clinical wound treatment.