Generation of monoclonal antibody Fab fragments to parathyroid hormone related protein by phage display technology

Objective To develop monoclonal antibody Fab fragments using display technique.Methods The mouse IgG Fab genes of heavy and light chains were amplified from spleen cells of a parathyroid hormone relaed protein(PTHrP) immunized mouse.The combinatorial phage antibody library was prepared by inserting both heavy and light chain Fab genes into phagemid vector pComb3 and followed by infection of helper phage. The library was selected by purified recombinant PTHrP.Results The combinatorial phage antibody library was constructed successfully and the specific mouse Fabs to PTHrP were delected and expressed in E.coli.Conclusion The selected specific mouse Fabs can recognize PTHrP with high specificity.