Synergistic effect of sodium selenite and Pediococcus acidilactici on growth, intestinal bacterial counts, selenium bioavailability, hepatic enzymes and non‐specific immune response in rainbow trout ( Oncorhynchus mykiss )

Aquaculture NutritionVolume 26, Issue 1 p. 74-87 ORIGINAL ARTICLE Synergistic effect of sodium selenite and Pediococcus acidilactici on growth, intestinal bacterial counts, selenium bioavailability, hepatic enzymes and non-specific immune response in rainbow trout (Oncorhynchus mykiss) Masoud Kousha, Masoud Kousha Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, IranSearch for more papers by this authorSakineh Yeganeh, Corresponding Author Sakineh Yeganeh skyeganeh@gmail.com s.yeganeh@sanru.ac.ir orcid.org/0000-0001-7480-7315 Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Correspondence Sakineh Yeganeh, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Km 9 Darya Boulevard, P.O. Box: 578, Sari, Iran. Emails: skyeganeh@gmail.com; s.yeganeh@sanru.ac.irSearch for more papers by this authorAbdolsamad Keramat Amirkolaie, Abdolsamad Keramat Amirkolaie Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, IranSearch for more papers by this author Masoud Kousha, Masoud Kousha Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, IranSearch for more papers by this authorSakineh Yeganeh, Corresponding Author Sakineh Yeganeh skyeganeh@gmail.com s.yeganeh@sanru.ac.ir orcid.org/0000-0001-7480-7315 Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Correspondence Sakineh Yeganeh, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Km 9 Darya Boulevard, P.O. Box: 578, Sari, Iran. Emails: skyeganeh@gmail.com; s.yeganeh@sanru.ac.irSearch for more papers by this authorAbdolsamad Keramat Amirkolaie, Abdolsamad Keramat Amirkolaie Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, IranSearch for more papers by this author First published: 13 September 2019 https://doi.org/10.1111/anu.12968Citations: 6 [Correction added on 15 November 2019 after first publication: the reference (Adel, Lazado, Safari, Yeganeh, & Zorriehzahra, 2017) have been included in this version.] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Lactic acid bacteria (LAB) have a crucial role in inorganic selenium metabolism as well as their known desirable effects on fish. In this study, the synergistic effects of dietary sodium selenite and Pediococcus acidilactici on growth performance, intestinal bacterial counts, selenium bioavailability, hepatic antioxidant enzyme thioredoxin reductase activity and hepatic glycolytic enzyme activity that is hexokinase, phosphofructokinase and pyruvate kinase, and non-specific immune response such as serum lysozyme and complements C3, C4 and ACH50 activity in rainbow trout (Oncorhynchus mykiss) were investigated. Thus, a 3 × 3 factorial design experiment was conducted with nine purified diets including three levels of sodium selenite (0, 1 and 2 mg/kg) and three levels of P. acidilactici (0, 7 and 9 log CFU/g). After 8 weeks of feeding, weight gain and specific growth rate were increased by increasing dietary sodium selenite and P. acidilactici levels compared to control (p < .05), whereas feed conversion ratio and condition factor was decreased by increasing dietary sodium selenite and P. acidilactici amounts in comparison with control (p < .05). Survival rate was not significantly affected among the experimental treatments (p > .05). Total cultivable bacterial populations after 4 and 8 weeks of the feeding trial were not significantly different among the dietary treatments, while LAB levels were higher in P. acidilactici-fed groups than in control and selenium-fed groups (p < .05). Selenocysteine, methylselenocysteine and selenomethionine levels in the intestine of rainbow trout were increased by increasing the sodium selenite and P. acidilactici levels (p < .05), and selenocysteine was found the most selenium species in the trout intestine. The quantity of total selenium in the whole body, intestine, blood, liver and muscle of rainbow trout were increased by increasing the amounts of sodium selenite and P. acidilactici compared to control (p < .05). Hepatic thioredoxin reductase and hexokinase activity were increased by increasing dietary selenium and P. acidilactici levels in comparison to control (p < .05), whereas phosphofructokinase and pyruvate kinase activity in the liver of rainbow trout were not significantly different between the dietary treatments. Serum lysozyme, complements C3, C4 and ACH50 activity were enhanced by increasing dietary selenium and P. acidilactici levels compared to control (p < .05). The most synergistic effects of dietary supplements on growth and metabolism of rainbow trout were obtained at 2 mg/kg sodium selenite and 7 log CFU/g P. acidilactici. The findings revealed the synergistic effect of dietary selenium and P. acidilactici on growth and metabolism in rainbow trout (O. mykiss). Citing Literature Volume26, Issue1February 2020Pages 74-87 RelatedInformation