适体
检出限
聚二甲基硅氧烷
基质(水族馆)
胶体金
材料科学
生物传感器
纳米技术
纳米颗粒
化学
线性范围
拉曼散射
拉曼光谱
色谱法
光学
地质学
物理
海洋学
生物
遗传学
作者
Afang Zhu,Shujat Ali,Yanhua Xu,Qin Ouyang,Quansheng Chen
标识
DOI:10.1016/j.bios.2020.112806
摘要
In this study, a sensitive biosensor was developed based on aptamer functionalized polydimethylsiloxane (PDMS) film for the detection of Staphylococcus aureus (S. aureus) using surface-enhanced Raman scattering (SERS) technology. Initially, the surface of PDMS film was chemically modified by piranha solution and 3-Aminopropyltriethoxysilane (APTES), and then AuNPs-PDMS film was prepared by coating gold nanoparticles (AuNPs) through electrostatic interaction. Next, the aptamers were immobilized on the AuNPs-PDMS membrane via gold-sulfur bond to form the capture substrate. Meanwhile, gold-silver core-shell nanoflowers ([email protected] NFs) modified with mercaptobenzoic acid (4-MBA) and aptamers were applied as a signal probe. In the presence of the target, the signal molecular probe and the capturing substrate specifically combined with the target and resulted in a sandwich structure “capture substrate-target-signal molecular probe”. Under the optimized experimental condition, the signal of 4-MBA at 1085 cm-1 was linearly related to the S. aureus concentration in the range of 4.3 × 10 cfu mL−1-4.3 × 107 cfu mL−1 (y = 326.91x-117.62, R2 = 0.9932) with a detection limit of 13 cfu mL−1. The method was successfully applied to spiked actual samples and a 92.5–110% recovery rate was achieved.
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