化学
氧化应激
生物物理学
体内
荧光
活性氧
谷胱甘肽
氧化磷酸化
荧光寿命成像显微镜
生物化学
酶
量子力学
生物
物理
生物技术
作者
Xiaopeng Yang,Xin Han,Yongru Zhang,Jianfei Liu,Jun Tang,Di Zhang,Yufen Zhao,Yong Ye
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2020-07-31
卷期号:92 (17): 12002-12009
被引量:50
标识
DOI:10.1021/acs.analchem.0c02509
摘要
Mercury (Hg) is considered an extremely toxic heavy metal which is extremely harmful to both the human body and environment. In addition, Hg2+-induced oxidative stress also exerts a crucial role to play in pathophysiological mechanisms of mercury toxicity. Thus, efficient and specific fluorescent probes for imaging Hg2+-induced oxidative stress are necessary. In the present study, we rationally design a novel Hg2+-activated and ICT-based NIR emission fluorescent probe NIR-HO for sequentially monitoring the ONOO– level with a “dual-key-and-lock” strategy. The probe NIR-HO showed rapid response and excellent specificity and sensitivity for the detection of Hg2+ and ONOO– in vitro. Cell imaging demonstrated that Hg2+-induced oxidative stress was involved in ONOO– upregulation. Also, GSH, NAC, and EDTA were employed as excellent detoxifying drugs against Hg2+-induced toxicity. Moreover, the probe NIR-HO was successfully used for imaging Hg2+ and ONOO– in vivo. In brief, NIR-HO provides a simple and powerful approach which can be used to image Hg2+-induced oxidative stress in the pathological environment.
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