Sustainable Synthesis of Ionic Liquid-Functionalized Zinc Oxide Nanosheets (IL@ZnO): Evaluation of Antibacterial Potential Activity for Biomedical Applications

Zinc oxide (ZnO)-derived materials exhibit unique antibacterial, antifungal, and photochemical activities and are widely used in antibacterial formulations. In this work, ZnO nanosheets were prepared by green and cost-effective synthesis via a hydrothermal method, and the prepared ZnO nanosheets were further functionalized with an eco-friendly ionic liquid (IL). Thus, a sustainable approach was established to synthesize ZnO nanosheets. The functionalization of ZnO with the synthesized IL was fully characterized by advanced spectroscopic and microscopic techniques. The prepared ionic liquid-functionalized ZnO (IL@ZnO) showed self-organized layered-sheet arrangements caused by the intercalation of the IL onto the surface of ZnO nanosheets as revealed by scanning electron microscopy (SEM). The design of the IL comprised a carboxylic acid moiety for functionalization onto the surface of ZnO, whereas the hydrophobicity was tuned through the incorporation of a long alkyl chain. The developed IL@ZnO material was also tested against both Gram-positive and Gram-negative pathogenic bacteria for potential antibacterial activity by colony-forming unit (CFU) and minimum inhibitory concentration tests. The results revealed that the IL@ZnO exhibits significant antibacterial activity against tested strains. In particular, potent activity was observed against the Gram-positive skin-specific Staphylococcus aureus bacteria strain. The mechanism of bactericidal activity against bacteria was also explored along with the cytotoxicity toward mammalian cells, which reveals that the IL@ZnO is nontoxic in nature. To utilize the developed material owing to its bactericidal activity for practical applications, the IL@ZnO was fabricated onto the surface of cotton fabric, and its surface morphology was examined by SEM; the activity of IL@ZnO-treated cotton fabric was evaluated by the zone of inhibition assay. Additionally, the IL@ZnO-treated cotton fabric exhibited remarkable stability along with significant hydrophobicity and breathability and thus can be utilized as a biomaterial for biomedical applications, especially in medical masks, for reducing the risk of transmission of infectious diseases.