Bifunctional Pdots-Based Novel ECL Nanoprobe with Qualitative and Quantitative Dual Signal Amplification Characteristics for Trace Cytokine Analysis

In this work, a novel methodology to design bifunctional ECL-luminophores with self-enhanced and TSA-amplified characteristics was proposed for improving the sensing performance of ECL-immunosensor toward trace cytokine analysis. Thanks to the qualitative- and quantitative- dual signal amplification technique, the as-prepared ECL biosensor demonstrated excellent detection performance. By analyzing the prospective cytokine biomarkers (IL-6), the ECL immunosensor exhibited a broad examination range with quite low detection limit and quite high selectivity, which was far superior to commercial ELISA kits and ever reported works. In particular, the novel ECL nanoprobe developed here could also be applied to monitor other immune toxicities or disease-related cytokines by using the respective antibodies corresponding to these targets. Moreover, the concept and construction strategy of self-amplified ECL-luminophores presented here could be further extended to design a series of Pdots-derived multicolored ECL probes to meet the needs of multipathway detection applications.