Polyoxometalate-Based Metal–Organic Frameworks as the Solid Support to Immobilize MP-11 Enzyme for Enhancing Thermal and Recyclable Stability

The immobilization of enzymes has received much attention. Metal–organic framework (MOF) as the adsorbent for enzyme encapsulation provides an effective strategy. However, the encapsulation efficacy is not dependent solely on the specific surface area. Though leading into appropriate substrate with negative charge would enhance the encapsulation efficacy. Polyoxometalates (POMs) as the electron sponge would donate electrons without any structural change. In this study, Keggin-type phosphotungstic acid (PW12) was encapsulated in Zirconium metal–organic framework (PW12@UiO-67) as a heterogeneous adsorbent for the encapsulation of enzyme. Our following data proved that this composite cluster could enhance the adsorption of enzyme and the stability of MP-11 was then significantly improved after immobilization.