In this paper, a new UV adhesive bonding technique for the rapid and low-cost bonding of plastic biochips at room temperature has been developed and characterized. The spreading characteristics of biocompatible UV adhesive have been explored to optimize screen-printing around microchannels to achieve excellent sealing of microfluidic structures. We also have investigated the effect of surface modification on the bond strength of the UV adhesive. The results show that hydrophilic surface treatment enhances the bond strength, whereas hydrophobic treatment drastically reduces the bond strength.