电化学发光
化学
核酸酶
荧光
纳米团簇
检出限
接受者
生物传感器
费斯特共振能量转移
鲁米诺
发光
分子信标
光化学
生物物理学
分析物
线性范围
纳米探针
小RNA
共振(粒子物理)
核酸
信号(编程语言)
分析化学(期刊)
化学发光
DNA
色谱法
生物化学
光学
有机化学
凝聚态物理
物理
粒子物理学
作者
Xiao-Lei Huo,Nan Zhang,Hui Yang,Jing-Juan Xu,Hong-Yuan Chen
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2018-10-25
卷期号:90 (22): 13723-13728
被引量:87
标识
DOI:10.1021/acs.analchem.8b04141
摘要
In this study, we proposed a dual-wavelength electrochemiluminescence resonance energy transfer (ECL-RET) ratiometric sensor combined with duplex-specific nuclease (DSN)-assisted target recycling amplification to detect microRNAs (miRNAs). Due to the perfect overlapping of spectra, the gold-nanoparticle-luminol-layered-double-hydroxides (Au NP-luminol-LDH) nanocomposite and gold nanoclusters (Au NCs) exhibited excellent ECL-RET effect with high efficiency. The Au NP-luminol-LDH donor exhibits a strong and stable ECL emission at the wavelength peak of 440 nm, while the Au NC acceptor has an emission peak at 620 nm. Upon the introduction of DSN and target miRNAs, the specific DNA-RNA binding and nuclease cleaving could trigger the detachment of capture Au NCs-DNA from the surface of Au NP-luminol-LDH, resulting in an increased ECL signal of Au NP-luminol-LDH and a decreased fluorescence signal of Au NCs. By measuring the ratio of optical signals at 440 and 620 nm, the designed sensor provided a quantitative readout proportional to the target miRNAs concentration in the range of 10 aM to 100 pM with a lower detection limit (LOD) of 9.4 aM.
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