哈卡特
生物相容性
伤口愈合
生物医学工程
真皮成纤维细胞
体内
成纤维细胞
壳聚糖
角质形成细胞
体外
MTT法
化学
再生(生物学)
活力测定
人体皮肤
材料科学
细胞生物学
外科
医学
生物化学
生物
生物技术
有机化学
遗传学
作者
C Intini,Lisa Elviri,Jaydee D. Cabral,Sonya Mros,Carlo Bergonzi,Annalisa Bianchera,Lisa Flammini,Paolo Govoni,Elisabetta Barocelli,Ruggero Bettini,Michelle McConnell
标识
DOI:10.1016/j.carbpol.2018.07.057
摘要
The fabrication of porous 3D printed chitosan (CH) scaffolds for skin tissue regeneration and their behavior in terms of biocompatibility, cytocompatibility and toxicity toward human fibroblasts (Nhdf) and keratinocytes (HaCaT), are presented and discussed. 3D cell cultures achieved after 20 and 35 days of incubation showed significant in vitro qualitative and quantitative cell growth as measured by neutral red staining and MTT assays and confirmed by scanning electron microphotographs. The best cell growth was obtained after 35 days on 3D scaffolds when the Nhdf and HaCaT cells, seeded together, filled the pores in the scaffolds. An early skin-like layer consisting of a mass of fibroblast and keratinocyte cells growing together was observed. The tests of 3D printed scaffolds in wound healing carried out on streptozotocin-induced diabetic rats demonstrate that 3D printed scaffolds improve the quality of the restored tissue with respect to both commercial patch and spontaneous healing.
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