Influence of matrigel on biodistribution studies in cancer research.

作者
Markus Wolf
出处
期刊:PubMed [National Institutes of Health]
卷期号:63 (1): 43-8 被引量:5
链接
标识
摘要

Matrigel matrix is widely used for supporting tumour implantation. However, some tumour cells are unable to degrade matrigel matrix resulting in residual matrigel at the time point of the biodistribution study or therapy experiment. In vitro cell uptake of tumour affine compounds into tumour cells embedded in matrigel was compared with matrigel free tumour cells. Matrigel accumulation exceeded cellular uptake of the tumour affine peptides. This suggests that matrigel might have an influence on the acquired biodistribution data when it is still present at the time point of the study. A quantitation of residual matrigel in tumour explants fourteen days after tumour implantation with a matrigel-tumour cell mixture showed that the overall matrigel content in the case of MCF-7 and AR42J tumours was about 23%. In order to evaluate the extent of accumulation of compounds in matrigel, nude mice bearing either tumours, tumour-matrigel-mixtures or matrigel alone received intravenous injections of fluorophor tagged tumour specific peptides. Fluorescence microscopy of cryosectioned matrigel, matrigel-tumour mixtures and tumour explants showed that the labelled compounds were matrigel associated and tumour cell associated with a higher fluorescence intensity in matrigel. In summary, matrigel matrix can influence biodistribution studies. It leads to believe in a higher tumour accumulation. Therefore, either a number of control experiments or the separation of matrigel from the tumour is necessary in order to obtain correct biodistribution data.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
今后应助Copyright采纳,获得10
刚刚
从容的胡萝卜完成签到,获得积分10
刚刚
爱吃果冻发布了新的文献求助10
刚刚
青柠发布了新的文献求助10
1秒前
2秒前
2秒前
3秒前
xxx发布了新的文献求助10
3秒前
3秒前
4秒前
KjLumos完成签到,获得积分10
5秒前
zoie0809完成签到,获得积分10
6秒前
Hello应助Mark采纳,获得10
6秒前
7秒前
乐观莞发布了新的文献求助10
7秒前
小二郎应助Yuu采纳,获得10
7秒前
可爱的函函应助zhgj采纳,获得10
7秒前
7秒前
南冥发布了新的文献求助10
7秒前
8秒前
8秒前
cdercder应助oleskarabach采纳,获得10
8秒前
负责可愁发布了新的文献求助10
10秒前
YEEze发布了新的文献求助10
10秒前
11秒前
11秒前
神勇从波发布了新的文献求助10
11秒前
11秒前
mengdewen发布了新的文献求助10
12秒前
13秒前
吉不得发布了新的文献求助10
13秒前
传奇3应助花开那年采纳,获得10
13秒前
龙飞凤舞完成签到,获得积分0
14秒前
14秒前
搞怪元彤完成签到,获得积分10
14秒前
迷路小丸子完成签到,获得积分10
14秒前
14秒前
15秒前
15秒前
赘婿应助Sea_U采纳,获得10
16秒前
高分求助中
Principles of Economics, 11th Edition 10000
University Physics with Modern Physics, 16th edition 10000
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Tanning Chemistry: The Science of Leather (2nd Edition) 2000
Development of a Bridge Weigh-In-Motion System: A technology to convert the bridge response to the passage of traffic into data on vehicle configurations, speeds, times of travel and weights 1000
Molecular Mechanisms of Photosynthesis, 4th Edition 1000
Organic Reactions, Volume 116 1000
热门求助领域 (近24小时)
化学 材料科学 医学 生物 纳米技术 工程类 有机化学 化学工程 生物化学 计算机科学 内科学 物理 复合材料 催化作用 细胞生物学 无机化学 光电子学 物理化学 电极 基因
热门帖子
关注 科研通微信公众号,转发送积分 7259677
求助须知:如何正确求助?哪些是违规求助? 8881558
关于积分的说明 18766521
捐赠科研通 6939772
什么是DOI,文献DOI怎么找? 3201645
关于科研通互助平台的介绍 2375437
邀请新用户注册赠送积分活动 2177391