农杆菌
胞质分裂
转化(遗传学)
有丝分裂
植物细胞
细胞分裂
细胞生物学
绿色荧光蛋白
生物
师(数学)
细胞
遗传学
基因
数学
算术
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 17-25
被引量:12
标识
DOI:10.1007/978-1-4939-3142-2_2
摘要
The continuing analysis of plant cell division will require additional protein localization studies. This is greatly aided by GFP-technology, but plant transformation and the maintenance of transgenic lines can present a significant technical bottleneck. In this chapter I describe a method for the Agrobacterium-mediated genetic transformation of tobacco BY-2 cells. The method allows for the microscopic analysis of fluorescence-tagged proteins in dividing cells in within 2 days after starting a coculture. This transient transformation procedure requires only standard laboratory equipment. It is hoped that this rapid method would aid researchers conducting live-cell localization studies in plant mitosis and cytokinesis.
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