Cytotoxicitic triterpene glycosides from sea cucumber Holothuria scabra Jaeger

Aim To study the bioactive triterpene glycosides of sea cucumber Holothuria scabra Jaeger.Methods Triterpene glycosides in the sea cucumber were isolated and purified by column chromatography on DA-101,silica gel,reversed-phase silica and reversed HPLC.Their chemical structure were identified on the basis of chemical evidence and spectral data.The cytotoxicities of the compounds obtained were tested with HL-60 cells and MOLT-4 cancer cells.Results Five triterpene glycosides were obtained from the sea cucumber studied and their structures were identified as scabraside C(1),holothurin A1(2),echinoside A(3),24-dehydroechinoside A(4) and holothurin A(5).Their inhibition activities on HL-60,MOLT-4 and A-549 cells were significant.Conclusion Compounds 15 are obstained from Holothuria scabra Jaeger for the first time.All glycosides good show antitumor activity in vitro.