Cloning and expressing of tannase gene from {\sl Aspergillus oryzae} in {\sl Aspergillus niger} ST31

It is the first time to research about expression of heterologous tannase gene using Aspergillus niger expression system. The gene encoding Tannase was cloned from A. oryzae by PCR. After tannase was sequenced, expression vector ANEP2-SP2-tan was constructed and was transformed into A. niger ST31 by protoplast transformation. Then, assaying of recombinant tannase activity were done. So, a novel recombinant Aspergillus strain containing tannase gene was obtained. Maximal tannase activity was 104.02 U/ml. It is as 2 to 3 times as the original strain. Based on the study, high efficient transformation system of A. niger is constructed and applied latitude of A. niger expression system was broadened. It is also useful for further working in research of new enzymes.