Method of HPLC for fipronil was studied in this article. Fipronil was determined by HPLC with UV detector, ODS column and acetonitrile+methanol+water as mobile phase, the results was satisfactory. The determination results of HPLC showed that the relative standard deviations was 0.42% and average spiked recoveries of fipronil were within 100.5%–104.3%.