脾脏
生物
流式细胞术
免疫学
环磷酰胺
白浆
免疫抑制
红浆
地塞米松
化疗
内分泌学
遗传学
作者
Adriano Alves Pereira,De Araújo Rs,Brito Rr,Xavier Jg,Bondan Ef,Josivânia Soares Pereira,Maria Anete Lallo
出处
期刊:PubMed
日期:2008-09-01
卷期号:17 Suppl 1: 292-5
被引量:1
摘要
Immunodeficient animals are important research models for studies in parasitology, oncology and immunology. Immunosuppressive drugs have been experimentally used to obtain a state of immunodeficiency in mice. This investigation aimed to quantify the circulating T and B cells of mice treated with the immunosuppressive agents dexamethasone (Dx), cyclosporine (CsA) and cyclophosphamide (CY), as well as to observe the behaviour of lymphocytic populations in the spleen of these animals. Blood samples were collected for counting the total peripheral blood leukocytes and T and B lymphocytes using flow cytometry. Total leukocytes of mice treated with the three drugs during all study showed a significant decrease when compared to the results of the control group. The proportion of B and T lymphocytes from the treated animals also decreased significantly. Spleen sections revealed a moderate decrease in the cellularity of the white pulp and the development of lymphocyte apoptosis in mice from groups treated with CY and Dx. Results showed that the proposed experimental models demonstrated to be suitable for studies of murine immunodeficiency.
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