Expression and localization of GFP-HPV18 E2 and its mutants fusion proteins in eukaryotic cells

Objective:To study expression and localization of GFP-HPV18 E2 and its TAD or DBD fusion proteins in macrophages.Methods:The plasmid of pEGFP-C1/ E2,pEGFP-C1/TAD,pEGFP-C1/DBD or pEGFP-C1 was transfected into macrophages,respectively.Their expression and localization were observed with fluorescent microscope.Western blot was used to analyze GFP or its fusion proteins by the antiGFP antibody as the first antibody.Results:Fluorescence could be observed in the whole cells transfected by pEGFP-C1,mainly in the nuclei of the cells transfected by pEGFP-C1/E2,but only in the nuclei of the cells transfected by pEGFP-C1/DBD while only in the cytoplasma of the cells transfected by pEGFP-C1/TAD.Western blot showed that the expression of GFP,GFP-E2,GFP-DBD,GFP-TAD fusion proteins.Conclusion:GFP-HPV18 E2 and its mutants could express in macrophages.GFP-E2 fusion protein locate mainly in nuclei,while GFP-DBD fusion protein locate completely in nuclei and GFP-TAD fusion protein locate completely in cytoplasma.