A new process for isolating Lleucine from corn gluten acid hydrolysate was proposed.Corn gluten was hydrolyzed with hydrochloric acid.After removing portion of the acid by concentration,3,4dimethylbenzenesulfonic acid was added into the hydrolysate to precipitate Lleucine.Pure Lleucine could be obtained by amminolysing the resulte precipitate and by the succeedent purification procedures.The yield of Lleucine was 3.2%.The product quality was in accord with the Japanese Standards.