[Objective] To explore a rapid and accurate method for detection requirements of food microbiology laboratory.[Method]The four methods Baird-Parker plate,MPN,Staphylococcus aureus CHROMagar chromogenic media and PetrifilmTMtest paper method were compared in quantitative detection of Staphylococcus aureus in food.[Result]The results showed that all the four methods exhibited high selectivity for S.aureus.Although the MPN,chromogenic media and PetrifilmTMtest paper methods were slightly more sensitive than Baird-Parker plate.The detection rates of the four methods showed no significant difference in S.aureus.It is suggested that chromogenic medium and PetrifilmTMtest paper method may be an auxiliary means for daily inspection of S.aureus in order to improve detective sensitivity and shorter testing time.[Conclusion]The study can accumulate experience for research and application of food detection method,provide a certain reference for detection work of Staphylococcus aureus in the future.