Combined fludarabine and cyclophosphamide pretreatment alleviated acute graft-versus-host disease

Objective To explore the effect of fludarabine combined with cyclophosphamide pretreatment on adoptive lymphocyte engrafment as well as the incidence of acute graft-versus-host disease(GVHD). Methods Female BALB/C mice of 8-10 weeks and male BALB/C×C57BL/6→F1 mice of 8-10 weeks were divided into five groups according to the pre-treatment methods(n=12): fludarabine combined with cyclophosphamide group,fludarabine alone group,cyclophosphamide alone group,total body irradiation(TBI) group and control group.After the pretreatment,5×107 donor mouse spleen cells were engrafted,and the chimerism of doner lymphocytes,the survival time and the manifestations of acute GVHD were observed. Results Compared with the TBI group,the fludarabine and cyclophosphamide group had a similar splenocytes chimeric rate,while the incidence of acute GVHD were significantly decreased(P0.05). Conclusions Combination of fludarabine and cyclophosphamide can promote adoptive allogeneic lymphocytes engrafment while alleviate acute graft-versus-host disease.