Differently expressed genes in adipocyte 3T3-L1 cell studied with cDNA microarray

Objective:To screen the differently expressed genes during cell differentiated of adipocyte 3T3-L1 using cDNA microarray.Methods:3T3-L1 cells were cultured,induce them differentiated,isolate total RNA.Reversely transcripted to cDNAs with fluorescent dUTP to prepare the target sequences,then the target sequences were hybridized to cDNA micmarray of 45038 human genes.After washing,the cDNA microarray were scanned for the fluorescent signals and showed differences between 2 kinds of cells.Results:Among 45038 geneprobes,the expression level of 640 genes changed,38 genes showed dramatically differential expressed profile with 24 upregulated and 14 downregulated genes.The differential expressed genes were related with apoptosis,pinto or antioncogene,cell signal conduct,and cytoskeleton,etc.These genes might be related with the pathogenesis of adipocyte differentiation.Conclusion:The investigations based on cDNA micmarray can sequence related genes in adipocyte differentiation,and may provide a new idea in studying pathogenesis of adipocyte differentiation.