Experimental study on the levels of IL-1α、IL-1β and IL-11 secreted by bone marrow stromal cells of patients with psoriasis

To reveal the abnormity of bone marrow microenvironment of patients with psoriasis by comparing the level of IL-1α,IL-1β and IL-11 secreted by bone marrow stromal cells of patients and control individuals,separate bone marrow mononuclear cells of patients and controls were isolated by density gradient centrifugation.and the bone marrow stromal cells were cultivated by the adherence method.When the cells were cultured for 3 passages and another 72 hours,bone marrow stromal cells and the supernatant liquid were collected.the phenotypes of cells were identified by flow cytometry and the level of IL-1α,IL-1β and IL-11 were assayed by ELISA kits.The results showed that the purity of bone marrow stromal cells was over 90%,and the level of IL-1α and IL-1β secreted by bone marrow stromal cells of patients was lower than that of control(P0.05).However,the level of IL-11 showed no significant difference between these two groups(P0.05).It is concluded that abnormality exists in the secretion of IL-α and IL-β by bone marrow stromal cells in patients with psorriasis,indicating an abnormal bone marrow microenviorment in this kind of patients.