基因座(遗传学)
生物
DNA测序
结构变异
单倍型
计算生物学
遗传学
基因组
等位基因
基因
作者
Quint P. Hottentot,Max van Min,Erik Splinter,Stefan J. White
出处
期刊:Methods in molecular biology
日期:2016-11-08
卷期号:: 185-196
被引量:33
标识
DOI:10.1007/978-1-4939-6442-0_13
摘要
Despite developments in targeted and whole-genome sequencing, the robust detection of all genetic variation, including structural variants, in and around genes of interest and in an allele-specific manner remains a challenge. Targeted locus amplification (TLA) is a cross-linking-based technique that generates complex DNA libraries covering >100 kb of contiguous sequence surrounding one primer pair complementary to a short locus-specific sequence. In combination with next-generation sequencing, TLA enables the complete sequencing and haplotyping of targeted regions of interest. Here we outline the basis of TLA, together with a detailed protocol of the technique.
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