已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Interleukin 6 Plays a Role in the Migration of Magnetically Levitated Mesenchymal Stem Cells Spheroids

间充质干细胞 归巢(生物学) 细胞生物学 趋化性 骨髓 球体 基质金属蛋白酶 细胞迁移 利基 生物 化学 免疫学 细胞 细胞培养 生物化学 生态学 受体 遗传学
作者
Jake Casson,Sam O’Kane,Carol‐Anne Smith,Matthew J. Dalby,Catherine C. Berry
出处
期刊:Applied sciences [Multidisciplinary Digital Publishing Institute]
卷期号:8 (3): 412-412 被引量:13
标识
DOI:10.3390/app8030412
摘要

Mesenchymal stem cells (MSCs) reside quiescently within a specialised ‘niche’ environment in the bone marrow. However, following appropriate signalling cues, MSCs mobilise and migrate out from the niche, typically toward either sites of injury (a regenerative response) or toward primary tumours (an intrinsic homing response, which promotes MSCs as cellular vectors for therapeutic delivery). To date, very little is known about MSC mobilisation. By adopting a 3D MSC niche model, whereby MSC spheroids are cultured within a type I collagen gel, recent studies have highlighted interleukin-6 (IL-6) as a key cytokine involved in MSC migration. Herein, the ability of IL-6 to induce MSC migration was further investigated, and the key matrix metalloproteinases used to effect cell mobilisation were identified. Briefly, the impact of IL-6 on the MSC migration in a two-dimensional model systems was characterised—both visually using an Ibidi chemotaxis plate array (assessing for directional migration) and then via a standard 2D monolayer experiment, where cultured cells were challenged with IL-6 and extracted media tested using an Abcam Human MMP membrane antibody array. The 2D assay displayed a strong migratory response toward IL-6 and analysis of the membrane arrays data showed significant increases of several key MMPs. Both data sets indicated that IL-6 is important in MSC mobilisation and migration. We also investigated the impact of IL-6 induction on MSCs in 3D spheroid culture, serving as a simplistic model of the bone marrow niche, characterised by fluorescently tagged magnetic nanoparticles and identical membrane antibody arrays. An increase in MMP levels secreted by cells treated with 1 ng/mL IL-6 versus control conditions was noted in addition to migration of cells away from the central spheroid mass.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
忧郁妙之发布了新的文献求助10
1秒前
在水一方应助风信子采纳,获得10
8秒前
十一一完成签到 ,获得积分10
12秒前
悟格完成签到,获得积分10
12秒前
moxin关注了科研通微信公众号
12秒前
春和景明完成签到 ,获得积分10
13秒前
高大凌寒应助殷勤的紫槐采纳,获得200
15秒前
16秒前
17秒前
大模型应助诚心淇采纳,获得10
17秒前
科研通AI5应助热情的老虎采纳,获得10
17秒前
18秒前
羽寞完成签到,获得积分10
19秒前
77完成签到 ,获得积分20
19秒前
ytrewq完成签到 ,获得积分10
21秒前
21秒前
777发布了新的文献求助10
21秒前
天天天蓝发布了新的文献求助10
22秒前
bc应助酷酷的惜海采纳,获得30
23秒前
酷波er应助科研通管家采纳,获得10
24秒前
李爱国应助科研通管家采纳,获得10
24秒前
pluto应助科研通管家采纳,获得10
24秒前
SYLH应助科研通管家采纳,获得10
24秒前
深情安青应助科研通管家采纳,获得10
24秒前
NexusExplorer应助科研通管家采纳,获得10
24秒前
SYLH应助科研通管家采纳,获得10
25秒前
SYLH应助科研通管家采纳,获得10
25秒前
SYLH应助科研通管家采纳,获得10
25秒前
SYLH应助科研通管家采纳,获得10
25秒前
完美世界应助科研通管家采纳,获得10
25秒前
完美世界应助科研通管家采纳,获得10
25秒前
Owen应助科研通管家采纳,获得10
25秒前
橘子圭令完成签到,获得积分10
25秒前
pluto应助科研通管家采纳,获得10
25秒前
SYLH应助科研通管家采纳,获得10
25秒前
共享精神应助科研通管家采纳,获得10
25秒前
英俊的铭应助司空靖琪采纳,获得10
27秒前
bc应助冷傲老九采纳,获得150
31秒前
莫得感情完成签到 ,获得积分20
31秒前
可爱的函函应助完美的火采纳,获得10
34秒前
高分求助中
Technologies supporting mass customization of apparel: A pilot project 600
Izeltabart tapatansine - AdisInsight 500
Chinesen in Europa – Europäer in China: Journalisten, Spione, Studenten 500
Arthur Ewert: A Life for the Comintern 500
China's Relations With Japan 1945-83: The Role of Liao Chengzhi // Kurt Werner Radtke 500
Two Years in Peking 1965-1966: Book 1: Living and Teaching in Mao's China // Reginald Hunt 500
Epigenetic Drug Discovery 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3815261
求助须知:如何正确求助?哪些是违规求助? 3359136
关于积分的说明 10400456
捐赠科研通 3076771
什么是DOI,文献DOI怎么找? 1690002
邀请新用户注册赠送积分活动 813551
科研通“疑难数据库(出版商)”最低求助积分说明 767674