产酸作用
厌氧消化
中层
嗜热菌
消化(炼金术)
生物
放线菌门
微生物学
废水
食品科学
化学
细菌
16S核糖体RNA
废物管理
色谱法
遗传学
甲烷
生态学
工程类
作者
Ying Wu,Erping Cui,Yiru Zuo,Weixiao Cheng,Christopher Rensing,Hong Chen
标识
DOI:10.1016/j.biortech.2016.03.086
摘要
The response of representative antibiotic resistance genes (ARGs) to lab-scale two-phase (acidogenic/methanogenic phase) anaerobic digestion processes under thermophilic and mesophilic conditions was explored. The associated microbial communities and bacterial pathogens were characterized by 16S rRNA gene sequencing. A two-phase thermophilic digestion reduced the presence of tetA, tetG, tetX, sul1, ermB, dfrA1, dfrA12 and intI1 exhibiting 0.1–0.72 log unit removal; in contrast, tetO, tetW, sul3, ermF and blaTEM even increased relative to the feed, and sul2 showed no significant decrease. The acidogenic phase of thermophilic digestion was primarily responsible for reducing the quantity of these genes, while the subsequent methanogenic phase caused a rebound in their quantity. In contrast, a two-phase mesophilic digestion process did not result in reducing the quantity of all ARGs and intI1 except for ermB and blaTEM. ARGs patterns were correlated with Proteobacteria and Actinobacteria during the two-phase anaerobic digestion.
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